The in vitro experiments were performed using cell viability assays, cell cycle assays, annexin-V/PI binding assays, Transwell migration assays, and wound-healing assays. Methods: Here, we used different concentrations of GSK-J4 or a combination of GSK-J4 and doxorubicin to treat human Cal-62, 8505C, and 8305C anaplastic thyroid cancer (ATC) cell lines. At present, there is no effective treatment for it. 6Department of Research and Development, Shenzhen Institute for Innovation and Translational Medicine, Shenzhen, Chinaīackground: Anaplastic thyroid cancer is the most aggressive thyroid cancer and has a poor prognosis.5RNA Biomedical Institute, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
4Key Laboratory of Stem Cells and Tissue Engineering (Sun Yat-sen University), Ministry of Education, Guangzhou, China.3Department of Breast Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou, China.2Institute of Urology of Shenzhen University, The Third Affiliated Hospital of Shenzhen University, Shenzhen Luohu Hospital Group, Shenzhen, China.1Department of Breast and Thyroid Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.Bo Lin 1†, Bing Lu 2†, I-yun Hsieh 1†, Zhen Liang 3, Zicheng Sun 1, Yang Yi 4, Weiming Lv 1*, Wei Zhao 4,5,6* and Jie Li 1*